Systems Biology of Gene Regulatory Elements

genome_center

Head of the Group

Prof. Dr. Nikolaus Rajewsky

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Secretariat

Alexandra Tschernycheff

87: Timoféeff-Ressovsky-House (Genomcentrum)

Room 1.08

Tel. +49 30 9406 2999

Fax. +49 30 9406 3068

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Research Interests and Approaches

The Rajewsky lab studies how RNA regulates gene expression. A past focus of the group was on mechanisms and functions of microRNAs. More recently, we have started to study the function of RNA binding proteins and long noncoding RNAs, including circular RNAs (circRNAs). We are using various model systems.

For understanding the role of noncoding RNA in (a) early development (b) stem cell biology and regeneration (c) function of neurons we use C. elegans, planaria, and mice, respectively. In collaboration with colleagues at the Charité, we are also analyzing human brain samples. Recently, we have established human brain organoids in the lab.

We integrate experimental (biochemistry, molecular biology) and computational (bioinformatics, physics, statistics) methods. We have developed several widely used methods (for example, recently, “MirDeep” (Friedlander et al Nature Biotechnology 2008, Friedlaender et al NAR 2012; miRNA target identification by chimera analyses (Grosswendt et al Mol. Cell 2014); fixation method for droplet based single cell sequencing (Alles et al BMC Biology 2017); “DistMap” for mapping sequenced single cells to correct positions within complex tissues (Karaiskos et al Science 2017)).  A few years ago we implemented and optimized “drop-seq” and are using it in many projects to sequence RNA from single cells in high throughput (e. g. Karaiskos et al Science 2017). We also develop methods to detect, quantify, image, and knockdown/knock out circRNAs (Memczak et al Nature 2013, Rybak-Wolf et al Mol. Cell 2014, Piwecka et al Science 2017).



Please see our projects for more information.

 

 

Figure: House 87: Timoféeff-Ressovsky-House (Genomcentrum)

Robert Rössle Str 10, 13092 Berlin-Buch

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