Microscopes

Olympus Fluoview 1000 Confocal Microscope

Laser lines:

405nm diode laser, 50mW

440nm diode laser, 25mW

Multi-Line Argon (458, 488, 515 nm)

515nm solid state laser, 25mW

559nm diode laser, 20mW

635nm diode laser, 20mW

Objectives:

UPLSAPO 4X / 0.16 NA, WD 13 mm

UPLSAPO 10X / 0.4 NA, WD 3.1 mm

UPLSAPO 20X / 0.75 NA, WD 0.6 mm

UPLSAPO 40X / 0.95 NA, WD 0.18 mm, oil

UPLSAPO 60XS / 1.3 NA, WD 0.3 mm, silicone oil

LUCPLFLN 20X / 0.45 NA, long working distance (6.6 – 7.8 mm)

LUCPLFLN 40X / 0,6 NA, long working distance (2.7 - 4 mm)

Features:

SIM Scanner - second scanning unit for simultaneous laser manipulation and imaging (FRAP, FLIP and photoactivation)

motorized stage (multi-area time-lapse imaging)

Incubation box to control temperature, humidity and CO2 (life cell imaging)

Z-drift compensation (ZDC, minimizes focus drift during time-lapse recording)

Eppendorf InjectMan NI 2 + FemtoJet (microinjection of fluorescent probes)

 

 

Olympus cell^R Imaging Station with cell^TIRF illuminator

Laser lines:

405nm diode laser, 50mW

455nm diode laser, 50mW

488nm solid state laser, 150mW

514nm diode laser, 50mW

561nm solid state laser, 150mW

640nm diode laser, 140mW

TIRF Objective: UAPON 150X / 1.45 NA, WD 0.07 mm

 

Features:

motorized 4 line TIRF system for simultaneous acquisition

motorized stage (multi-area time-lapse imaging)

Incubation box to control temperature, humidity and CO2 (life cell imaging)

Z-drift compensation (ZDC, minimizes focus drift during time-lapse recording)

Hamamatsu ImagEM EMCCD camera

Eppendorf InjectMan NI 2 + FemtoJet (microinjection of fluorescent probes)

PALM/dSTORM compatible

 

 

 

 

 

Picoquant Fluorescence Lifetime Imaging (FLIM) LSM upgrade

485 nm pulse laser LDH-D-C-485

PicoHarp 300 TCSPC Module

Single Photon Counting Module (SPAD)