In 1989 in a Nature paper, Stanley Fields and Ok-Kyu Song described a novel method to study protein-protein interactions in yeast. This technique became known as the Y2H system. The system is based on the simultaneous introduction of bait and prey plasmids into a mutant yeast strain. If bait and prey interact, a reporter gene is activated and enables growth of the yeast on selective medium. Clones that contain interacting bait and prey plasmids are made visible by a colour change reaction.
The research group Neuroproteomics has carried out pioneering protein-protein interaction studies, generating the first network for a protein central to a disease as well as the first genome-wide protein-protein interaction network (Goehler et al. 2004, Molecular Cell; Stelzl et al. 2005, Cell). The team apply their comprehensive know how in the field to offer screening services to the scientific community at the MDC and beyond.
Please contact us for further information or if you are interested in a screening.